The application of RT-PCR assay for the detection of Apple stem pitting virus and Apple stem grooving virus in four apple cultivars
نویسندگان
چکیده
منابع مشابه
شناسایی مولکولی و گروه بندی جدایه های دو ویروسapple stem grooving virus و apple stem pitting virus در استان اصفهان
چکیده سیب درختی (malus domestica borkh) متعلق به خانواده rosaceae از میوه های مهم و اقتصادی مناطق معتدله و سردسیری ایران است. ویروس ساقه شیاری سیب apple stem grooving virus (asgv) عضو تیپ جنس capillovirus و ویروس ساقه آبله ای سیب apple stem pitting virus (aspv) عضو تیپ جنس foveavirus متعلق به خانوادهbetaflexiviridae ، از مهمترین ویروس های بیماری زای سیب محسوب می شوند. آلودگی این دو ویروس درس...
15 صفحه اولGenetic Diversity of a Natural Population of Apple stem pitting virus Isolated from Apple in Korea
Apple stem pitting virus (ASPV), of the Foveavirus genus in the family Betaflexiviridae, is one of the most common viruses of apple and pear trees. To examine variability of the coat protein (CP) gene from ASPV, eight isolates originating from 251 apple trees, which were collected from 22 apple orchards located in intensive apple growing areas of the North Gyeongsang and North Jeolla Provinces ...
متن کاملThe RT-PCR identification and sequence analysis of Apple chlorotic leaf spot virus from apple cultivars in Jiaodong Peninsula, China
A set of specific primer pairs was utilized to detect Apple chlorotic leaf spot virus (ACLSV) from seven different apple cultivars in Jiaodong Peninsula via reverse transcription polymerase chain reaction (RT-PCR), and the sequence of ACLSV genome was analysed. The results indicate that: (1) High-purity total RNA could be successfully isolated using plant RNA rapid extraction kit. The ratios of...
متن کاملInfection of Apple by Apple Stem Grooving Virus Leads to Extensive Alterations in Gene Expression Patterns but No Disease Symptoms
To understand the molecular basis of viral diseases, transcriptome profiling has been widely used to correlate host gene expression change patterns with disease symptoms during viral infection in many plant hosts. We used infection of apple by Apple stem grooving virus (ASGV), which produces no disease symptoms, to assess the significance of host gene expression changes in disease development. ...
متن کاملPhylogenetic analysis and recombination events in full genome sequences of apple stem grooving virus.
Apple stem grooving virus (ASGV) is one of the most important viral pathogens infecting pome and stone fruit trees worldwide. In this study, with the complete nucleotide sequence of isolate ASGV-T47, which we generated, molecular variation and recombination in ASGV full genomic sequences worldwide were analyzed. ASGV-T47 shared 79.7-97.6% nucleotide identity with the other isolates worldwide an...
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ژورنال
عنوان ژورنال: Plant Protection Science
سال: 2012
ISSN: 1212-2580,1805-9341
DOI: 10.17221/4816-pps